Dataset on the genome sequence and biological characteristics of bacteriophage vB_RsoP_PBa1 infecting Ralstonia pseudosolanacearum GMI1000.

This dataset reports the isolation and genomic characterization of vB_RsoP_PBa1, a novel Caudoviricetes phage that infects Ralstonia pseudosolanacearum GMI1000. The phage was isolated and enriched using double-layer agar plaque assays. Genomic analysis revealed that vB_RsoP_PBa1 possesses a linear double-stranded DNA genome of 42,068 bp with a G+C content of 62.44%, encoding 53 predicted coding sequences, of which 18 were assigned putative functions. The annotated open reading frames include functional modules for genome packaging, virion assembly, host lysis (holin, lysozyme, and a helix-turn-helix domain-containing protein), host infection, genome replication, and phage integration. Phylogenetic analyses based on the proteomic and the terminase large subunit amino acid sequences demonstrated that vB_RsoP_PBa1 is most closely related to Ralstonia phage DU_RP_II (MF150911.1), with which it shares 78.8% average nucleotide identity. Overall, this dataset provides a valuable genomic resource for comparative phage genomics and supports the development of phage-based biocontrol strategies against bacterial wilt caused by R. pseudosolanacearum.