Rebecca Spanner, Ahmad H. Sallam, Yu Guo, Murukarthick Jayakodi, Axel Himmelbach, Anne Fiebig, Jamie Simmons, Gerit Bethke, Y. C. Lee, Luis Willian Pacheco Arge, Yinjie Qiu, Ana Badea, Michaël Baum, François Belzile, Roi Ben‐David, Robert Brueggeman, Austin J. Case, Luigi Cattivelli, Michael H. Davis, Christoph Dockter, Jaroslav Doležel, Antonín Dreiseitl, Ryan Gavin, Lior Glick, Stephan Greiner, Ruth Hamilton, Patrick M. Hayes, Scott Heisel, Cynthia A. Henson, Benjamin Kilian, Takao Komatsuda, Chengdao Li, Cheng Liu, Ramamurthy Mahalingam, Maren Maruschewski, Oadi Matny, Andreas Maurer, Klaus Mayer, Itay Mayrose, Matthew Moscou, Gary J. Muehlbauer, Youko Oono, Frank Ordon, Hakan Özkan, Aleš Pečinka, Dragan Perović, Klaus Pillen, Mohammad Pourkheirandish, Joanne Russell, Jan Šafář, Silvio Salvi, Miguel Sanchez‐Garcia, Kazuhiro Sato, Thomas Schmutzer, Uwe Scholz, Jeness C. Scott, Gurcharn S. Brar, Kevin P. Smith, Mark E. Sorrells, M. Spannagl, Nils Stein, Alessandro Tondelli, Roberto Tuberosa, James R. Tucker, T. Kelly Turkington, J. Valkoun, Ramesh Pal Singh Verma, Marcus A. Vinje, Maria von Korff, Jason G. Walling, Robbie Waugh, Roger P. Wise, Brande B. H. Wulff, Shengming Yang, Guoping Zhang, Peter L. Morrell, Martin Mascher, Brian J. Steffenson
To exploit allelic variation in Hordeum vulgare subsp. spontaneum, the Wild Barley Diversity Collection was subjected to paired-end Illumina sequencing at ∼9 × depth and evaluated for several agronomic traits. We discovered 240.2 million single nucleotide polymorphisms (SNPs) after alignment to the Morex V3 assembly and 24.4 million short (1 to 50 bp) insertions and deletions. A genome-wide association study of lemma color identified one marker-trait association (MTA) on chromosome 1H close to HvBlp, the cloned gene controlling black lemma. Four MTAs were identified for seedling stem rust resistance, including 2 novel loci on chromosomes 1H and 6H and one co-locating to the complex RMRL1-RMRL2 locus on 5H. The whole-genome sequence data described herein will facilitate the identification and utilization of new alleles for barley improvement.