FAIDR-Desi: interactive software for feature analysis and design of intrinsically disordered regions.

SUMMARY: FAIDR-Desi is a non-technical user interface for design of intrinsically disordered regions (IDRs) based on greedy optimization of sequence features (simple sequence statistics correlated with IDR function, including amino acid composition, residue patterning and short linear interaction motifs), using more features (127) than other IDR design approaches. FAIDR-Desi can be used to design synthetic IDR mimics to establish feature sufficiency for function, or to knock out features to show necessity for function, exemplified with experiments on the yeast Cox15 mitochondrial targeting signal. AVAILABILITY AND IMPLEMENTATION: Software is freely available on the web at https://moses-lab.github.io/faidr_desi/ and an archival version of the source code is available on Zenodo (https://doi.org/10.5281/zenodo.22036189). SUPPLEMENTARY INFORMATION: Supporting and supplementary information can be found on Zenodo (https://doi.org/10.5281/zenodo.22084467).