Scott Mastromatteo, Shalvi Chirmade, Delnaz Roshandel, Bhooma Thiruvahindrapuram, Zhuozhi Wang, Rohan V Patel, Wilson W L Sung, Amirhossein Hajianpour, Cheng Wang, Fan Lin, Katherine Keenan, Julie Avolio, Paul Eckford, Felix Ratjen, Canadian Cystic Fibrosis Gene Modifier Consortium, Lisa J Strug
SUMMARY: Pangenome variation graphs integrate multiple samples into a unified representation, mitigating the reference bias inherent to linear genomes. However, these graphs can be large and structurally complex. Existing visualization tools are each confined to a fixed scale of resolution, requiring researchers to switch between multiple tools to examine variation at different levels of detail. PangyPlot is an interactive pangenome browser designed for multi-scale exploration of reference variation graphs from full chromosome to nucleotide-level sequence segments. PangyPlot anchors navigation to linear reference coordinates, organizes variation into hierarchical bubble structures, and uses a force-directed layout engine for automatic node arrangement.
AVAILABILITY AND IMPLEMENTATION: An instance preloaded with data is available at https://pangyplot.research.sickkids.ca. Source code and documentation are openly available at https://github.com/strug-hub/pangyplot under the MIT License.