Tanveer Alam Khan, Sunil Mundra, Mayank Anand Gururani
Photosynthesis defines the upper limit of crop productivity, yet intrinsic inefficiencies in light capture, carbon fixation, and energy conversion constrain yield potential under variable environmental conditions. This review provides a mechanistic synthesis of recent advances in enhancing photosynthetic efficiency through molecular, biochemical, and biophysical strategies. We highlight key regulatory processes governing RuBisCO activity, ATP synthase function, photosystems, and light-harvesting complexes, together with emerging insights into redox modulation, photorespiration, and post-translational control. Innovations in genome editing, particularly CRISPR/Cas9, synthetic biology, and systems modeling, are accelerating the rational redesign of photosynthetic pathways to improve carbon assimilation and stress resilience. Engineering C₄ and CAM traits into C₃ crops, optimizing canopy light utilization, and modifying photoprotective and photorespiratory pathways demonstrate substantial potential to overcome long-standing biochemical and anatomical constraints. Integration of high-throughput phenotyping, multi-omics analysis, and computational modeling is now enabling predictive frameworks for photosynthetic improvement under fluctuating light, temperature, and water regimes. Coupling these molecular innovations with stress-tolerance traits such as enhanced antioxidant capacity and water-use efficiency offers a viable path toward climate-resilient, high-yield crops. Collectively, these advances illustrate how precise manipulation of photosynthetic processes can drive sustainable gains in agricultural productivity to meet future global food demand.