Ngnintedo Dominique, Ouété Nantchouang Judith Laure, Mebami Julia Ndifor, Ayimele Godfred Aponglen, Ngaitad Stanislaus Njinga, Dongmo Jumeta Johane Kevine, Lenta Djakou Bruno, Céline Henoumont, Sophie Laurent, Ache Roland Ndifor
Two novel lipids lentinoside A (6), [1-O-β-D-galactopyranosyl-(2S,3R,4E,8E)-2-[(2'R)-2-hydroxytricosanoylamino]-9-methyl-4,8-heptadecadiene-1,3-diol], and lentinamide A (5), [((2'R)-2-hydroxy-N-((2S,3S,4R)-1,3,4-trihydroxyheptatriacontan-2-yl)pentadecanamide)] were isolated from Lentinus sajor-caju (Fr.) Fr. 1838, alongside stearic acid (1), ergosterol (2), ergosterol endoperoxide (3), and asperglaucide (4). Antioxidant evaluation revealed that (3) (25.706%) and the extract (25.858%, IC50 = 0.188 mg/mL) exhibited DPPH• radical scavenging capacity, though lower than the reference ascorbic acid (21.26%, IC50 = 0.0621 mg/mL). In the ABTS•+ assay, (4) demonstrated the strongest activity (0.800 mg TE/g), followed by (6) (0.646 mg TE/g) and (5) (0.612 mg TE/g). FRAP assay revealed (5) had the highest ferric reducing capacity (0.982 ± 0.069 mg Fe(II)/g), followed by (4) (0.690 mg Fe(II)/g). In contrast, (2) (0.456 mg Fe(II)/g) and the extract (0.440 mg Fe(II)/g) displayed weak capacity. These findings highlight lipid and peptide contributions to the nutraceutical potential of L. sajor-caju and expands the chemical diversity of bioactive fungal metabolites.