Effects of dietary pyocyanin and green coffee powder supplementation on growth performance, antioxidant status, cecal microbiota, and meat quality of broiler chickens.

The present study investigated the effects of dietary supplementation with pyocyanin powder (PYP), a microbial-derived bioactive compound, and green coffee powder (GCP) as natural feed additives on growth performance, carcass traits, blood biochemical parameters, antioxidant status, meat quality, and cecal microbial populations in broiler chickens. A total of 300 one-week-old Ross 308 broiler chicks were assigned to five dietary treatments in a completely randomized design, with five replicates of 12 birds each, and reared until 35 days of age. The dietary treatments consisted of a basal diet (control), a basal diet supplemented with 1-2 g PYP/kg diet, and a basal diet supplemented with 1.30-2.60 g GCP/kg diet. Dietary supplementation with PYP improved selected growth performance traits, with birds fed 1 g PYP/kg diet reaching a final live body weight of 1,919.14 g and those receiving 2 g PYP/kg diet achieving the highest daily body weight gain during the grower period (51.34 g/day; P < 0.05), whereas feed intake and feed conversion ratio were not significantly affected. Supplementation with PYP also reduced serum creatinine and aspartate aminotransferase concentrations, while both PYP and GCP enhanced antioxidant status through increased glutathione-related indices and reduced malondialdehyde levels, which declined from 4.73 nmol/mL in the control group to 3.59 nmol/mL in birds supplemented with 2.60 g GCP/kg diet (P < 0.05). Furthermore, both additives improved selected meat quality characteristics by lowering thiobarbituric acid reactive substances (TBARS) and total volatile basic nitrogen (TVB-N) values. Cecal microbial populations were favorably modulated, as evidenced by increased lactic acid bacteria counts and reduced Salmonella spp. and Escherichia coli populations (P < 0.05). Overall, the findings suggest that dietary supplementation with PYP and GCP can improve selected productive, antioxidant, meat quality, and intestinal microbial characteristics in broiler chickens under the experimental conditions of this study, although further studies are needed to confirm their long-term safety and practical application.