Saeed Akkad, Eva W Wan, Alex Maddison, Jessica Rule Mcloughlin, Cindy Au-Yeung, Lloyd D Murphy, Greg L McNeil, Lianne I Willems
CDP-ribitol serves as an essential donor for d-ribitol-5-phosphate incorporation into bacterial and mammalian glycoconjugates. Here, we demonstrate the one-pot enzymatic synthesis of CDP-ribitol from the readily available precursor ribitol. We also explore the synthesis of bioorthogonally tagged derivatives and other CDP conjugates, thereby providing valuable new tools for glycoscience research.