◆ Chemical communications (Cambridge, England)2026-08-20
Simple and fast production of biologically active CXCL12/SDF-1 isoforms in E. coli using a Nanobody solubility partner.
Vanessa Wittek, Jonas Kaynert, Manuel M Vicente, Manuel H Taft, Christoph Garbers, Georg Zocher, Anja K Münster-Kühnel
原始摘要（英文原文）· Original abstract
CXCL12 is commonly produced by laborious inclusion-body refolding approaches. Here, we present a fast, scalable, and low-cost method for producing biologically active CXCL12/SDF-1 isoforms in E. coli within two days using a nanobody solubility partner, enabling simple and fast in-house production of the chemokines for multiple research applications.