Edan Habel, Martyna Judd, Thomas Huber, Nicholas Cox
原始摘要(英文原文)· Original abstract
19F-Gd3+ ENDOR is used to map the full conformational distribution of Trp42 in dihedral space in a de novo luminescent lanthanide-binding protein. By combining genetically encoded fluorination with regularisation modelling, we extract 19F-Gd3+ distance distributions, revealing a minor rotamer population proximal to the lanthanide, key for photoactivity.