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◆ Chemical communications (Cambridge, England)2026-08-30

Mapping conformational heterogeneity: 19F-Gd3+ ENDOR reveals otherwise hidden rotamer conformations.

Edan Habel, Martyna Judd, Thomas Huber, Nicholas Cox

原始摘要(英文原文)· Original abstract
19F-Gd3+ ENDOR is used to map the full conformational distribution of Trp42 in dihedral space in a de novo luminescent lanthanide-binding protein. By combining genetically encoded fluorination with regularisation modelling, we extract 19F-Gd3+ distance distributions, revealing a minor rotamer population proximal to the lanthanide, key for photoactivity.
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Mapping conformational heterogeneity: 19F-Gd3+ ENDOR reveals otherwise hidden rotamer conformations. — 科研速览 Science Skim