Application of aptamer nucleic acid test strips in the detection of tumor markers for colorectal cancer.

At present, the detection methods of tumor markers rely on ELISA, gene sequencing, and other technologies. However, these methods require specialized equipment and technical personnel, and the low concentrations of early tumor markers limit their sensitivity. This study developed a method for detecting colorectal cancer tumour markers. It uses a test strip and isothermal amplification to detect low concentrations of tumour markers. The test strip is easy to use and cost-effective. The test strip developed in this study can detect isothermal amplification products in 3 min, and the detection limit for PPIA is 5 ng mL-1. This method is expected to play a significant role in early cancer detection, providing an efficient and convenient approach for tumor marker detection.