Yuanliang Jiang, Qinglian Zeng, Linqiang Ma, Yifan He, Shuangshuang Zhu, Yunjie Xiong, Ying Jing, Qi Zhang, Shuangxin Qi, Jinbo Hu, Qifu Li, Shumin Yang
These findings indicate that different DF sources generate substrate-specific fecal microbial fermentation patterns in vitro. Among the tested substrates, AM produced a stronger SCFA- and H₂-associated fermentation response and was linked with selected microbial response patterns. Integrated correlation analysis suggested microbiota-associated co-variation between H₂ and SCFAs during DF fermentation, which requires further validation.
Idiopathic hyperaldosteronism (IHA) is a significant cause of secondary hypertension. Emerging evidence links the gut microbiota to endocrine and cardiovascular diseases. We integrated human genetics, multi-omics, and fecal microbiota transplantation (FMT) to investigate the association between IHA and gut microbiome. Two-sample Mendelian randomization identified seven bacterial taxa associated with IHA. Metagenomic analysis of 30 patients with IHA and 30 healthy control subjects revealed lower microbial diversity and depletion of protective genera identified by Mendelian randomization. Plasma metabolomics showed alterations in tryptophan metabolism and other metabolic pathways that overlapped with microbial functional changes. Tryptophan-derived metabolites correlated with the abundance of protective genera. FMT from patients with IHA increased serum aldosterone and elevated 5-hydroxy-L-tryptophan in germ-free mice. These findings support an association between gut microbiome dysbiosis, metabolic alterations, and aldosterone dysregulation, highlighting the gut microbiome as a potential target for the diagnosis and treatment of IHA.