Reconstitution of Membrane Proteins into Preformed Lipid Bilayers via LMNG-Mediated Auto-Insertion.

A major challenge for the successful implementation of an in vitro transporter assay is the reconstitution of a given transporter into a lipid bilayer for measurements of vectorial transport. Here, we present the application of lauryl-maltose neopentyl glycol LMNG-mediated direct membrane insertion, termed LAiR (LMNG Auto-insertion Reintegration), for its use on preformed lipid bilayers such as liposomes or planar bilayers. Its ease of use, the stability and tightness of the resulting reconstituted lipid bilayer membrane protein system make this novel approach an attractive choice for the establishment of robust membrane transport assays.