Sezgi Canaslan, Matthias Schmitz, Fabian Maass, Peter Hermann, Susana Da Silva Correia, Shuyu Zhang, Bastian Popper, Wiebke Möbius, Christoph van Riesen, Piero Parchi, Paul Lingor, Inga Zerr
Detection of alpha-synuclein (α-Syn) seeding activity in tear fluid (TF) offers a promising non-invasive biomarker for Parkinson's disease (PD). α-Syn seeding amplification assay (αSynSAA) detected seeding activity in 67% of PD-TF, while non-synucleinopathy samples remained negative. Electron microscopy of seeding-positive end products revealed fibrillar structures morphologically consistent with results of αSynSAA. αSynSAA effectively distinguished PD from controls and prion diseases based on seeding activity in TF.