Rémi Kinet, Joanna Sikora, Marie-Laure Arotçarena, Mélina Decourt, Éric Balado, Évelyne Doudnikoff, Sylvain Bohic, Marta Vesnaver, Anna Lovisotto, Marie-Laure Thiolat, Nathalie Dutheil, Claire Mazzocco, Karim Harhouri, Rémy Steinschneider, Séverine Ménoret, Laurent Tesson, Ignacio Anegón, Michele Morari, Miquel Vila, François Georges, Erwan Bezard, Pierre‐Olivier Fernagut, Benjamin Dehay
Mutations in the ATP13A2 gene were identified as the cause of Kufor-Rakeb syndrome (KRS), a juvenile-onset form of Parkinson's disease (PD). Developing relevant and predictive models for the rare PD forms is necessary to understand the pathological mechanisms and validate therapeutic strategies. Herein, we aimed to comprehensively characterize the first transgenic Atp13a2 knockout rat model. Behavioral assessment demonstrated specific developmental deficits in animals with deletion of Atp13a2. Further analysis revealed that Atp13a2 knockout rats displayed age-dependent fine motor skills deficits and impaired locomotor habituation similar to those observed in PD patients at the early stage of motor symptoms. In contrast, no change in the nigrostriatal integrity was observed. An extended investigation on heavy metals homeostasis, autophagy-related markers, and lipofuscin accumulation showed significant changes reminiscent of KRS. Finally, we tested whether inducing pathology by viral-mediated overexpression of human α-synuclein or human tyrosinase exacerbated the onset or extent of pathological changes. This Atp13a2 KO rat model could help better understand autophagy in PD pathogenesis and open new therapeutic validation opportunities.