Ksenia S. Anufrieva, Neda Shahriari, Ce Gao, Stephanie Pei Tung Yiu, Shideh Kazerounian, Rochelle L. Castillo, Jessica Liu, S. Prell, Kartik Bhamidipati, Khashayar Afshari, Lindsay Parmelee, Anastasia N. Kazakova, Erin Theisen, Teresa V. Bowman, Avery LaChance, William J. Crisler, Kimberly B. Hashemi, Ilya Korsunsky, Sizun Jiang, Mehdi Rashighi, Ruth Ann Vleugels, Kevin Wei
ABSTRACT Adult-onset dermatomyositis (DM) is an autoimmune inflammatory myopathy with distinct cutaneous manifestations and a strong malignancy association. Through comparative analysis with cutaneous lupus erythematosus (CLE), our integrated spatial and single-cell transcriptomics analysis revealed unique immune and stromal niches associated with DM subtypes. Unexpectedly, we found an association between cancer-associated DM skin lesions and the presence of dispersed immune infiltrates enriched with macrophages, CD8+ T cells, plasma cells, and B cells with preserved vascular architecture. In contrast, non-cancer associated DM skin exhibited dense myeloid cell infiltrates, including neutrophils, monocytes, and macrophages, with elevated expression of IL1B and CXCL10 localized near injured vascular endothelia. Cytokines produced by these myeloid infiltrates together with local tissue hypoxia triggered dramatic stromal remodeling, leading to loss of vascular-associated fibroblasts. In addition to the CXCL10+ myeloid signature, non-cancer-associated DM skin with pDC presence showed the emergence of specific cellular pairs: PD-L1-expressing mregDCs and activated Tregs expressing NFKB2 and TNF receptors. While both DM and CLE showed strong interferon signatures, DM uniquely displayed IFN-β expression. Together, our study provides the first comprehensive spatial mapping of immune and stromal cells in adult-onset DM.