Xiaotang Lu, Yuelong Wu, Peter H. Li, Tao Fang, Richard Schalek, Yaxin Su, Daniel R. Berger, Jeffery D. Carter, Sean Lynch, Daniel J. Schneider, Shashi Kumar Gupta, Viren Jain, Nebojša Janjić, Jeff W. Lichtman
Abstract Detergent-free immunolabeling has been proven feasible for correlated light and electron microscopy, but its application is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into recent approaches to delineate neural circuits via connectomics.