m6A-ARTR-DBiT for Spatial Mapping of m6A Epitranscriptome.

N 6-methyladenosine (m6A) is a widespread RNA modification that regulates RNA metabolism and gene expression, yet most sequencing methods rely on isolated RNA and therefore lose its spatial context. This In Focus article surveys methodological advances in transcriptome-wide m6A detection and highlights m6A-ARTR-DBiT, a spatial m6A profiling assay that leverages reverse-transcription-based detection and deterministic barcoding in tissue to map transcriptome-wide m6A distribution while preserving native tissue context. This platform enables researchers to investigate how m6A patterns vary across distinct tissue regions, developmental structures, and local gene regulatory networks, bridging the gap between spatially patterned epitranscriptomic deposition and gene regulation.