Yan-Mei Weng, Tian-Ze Li, Yun-Bao Ma, Hao-Wei Zhang, Ji-Jun Chen
Artemisiyneolides A-K (1-11), guaiane sesquiterpenoid-heptatriyne hybrids, were isolated from Artemisia sieversiana (Asteraceae). Their structures were elucidated using NMR, HRESIMS, ECD calculations, and X-ray diffraction analysis. Compounds 1-11 featured 5/5/7/5-fused ring frameworks bearing a 1,3,5-heptatriyne side chain by C-C linkages, which were biosynthetically generated by [4 + 2] Diels-Alder cycloadditions. The investigation of antihepatoma activity suggested that compound 4 exhibited significant inhibition on HepG2, Huh7, and SK-Hep-1 cell lines, with IC50 values of 22.8 ± 1.8, 31.7 ± 1.4, and 26.3 ± 3.0 μM, respectively.