Ningfang Kang, S Liu, X Q Li, Ruxiang Luo, W CHEN, Chongzhao Ran, Peng Wang, Jing Yang
: 7.7 ± 0.2 nM for EP-1, 4.6 ± 0.4 nM for EP-2), similar to Exatecan, but markedly reduced toxicity in AKR1C3-low and normal cells, and overcame sorafenib resistance. Cellular and zebrafish imaging confirmed a targeted release. In a mouse model, EP-2 displayed potent efficacy with significantly reduced systemic toxicity (MTD > 60 mg/kg) compared to Exatecan. These results nominate EP-2 as an ideal candidate for selective and safe tumor therapy, providing a new paradigm for biomarker-driven antitumor drug design.