Alexandra P Platt, Yannick J Bomble, Robert S Paton
When replacing expensive redox cofactors, making biomimetic cofactors with desirable thermostability and redox properties is only half of the equation; enzymes must also be able to use them. Ji and colleagues confront that trade-off by pairing the development of a thermostable carbocyclic cofactor with a dedicated regeneration enzyme for sustained biocatalysis.