Ruyu Li, Tingting Zhang, Lijie Zhou, Jingjing Feng, Longgang Wang, Haiyan Xiao
Traditional cancer therapies are limited by severe side effects and therapeutic challenges, while noble metal nanozymes offer promising enzyme-like therapeutic potential but remain constrained by poor aqueous stability. In this work, palladium-gold bimetallic nanoparticles (Pd3Au-LCLP NPs) with peroxidase-like activity were constructed using Leonurus cardiaca L. polysaccharide as both a reducing agent and stabilizing template, and further loaded with doxorubicin (DOX) to form Pd3Au-LCLP NPs-DOX. The resulting nanoplatform exhibited excellent water dispersibility, structural stability, anti-protein adsorption capability, and enhanced photothermal conversion performance. Under 808 nm laser irradiation, Pd3Au-LCLP NPs-DOX achieved a cumulative DOX release of 81% at pH 5.5. In vitro, it showed significant cytotoxicity against A549 and HeLa cells. In vivo, the tumor inhibition rate reached 82.6%. Overall, this combined therapeutic strategy integrating chemodynamic therapy (CDT), photothermal therapy (PTT), and chemotherapy (CT) demonstrated strong synergistic anti-tumor efficacy.