Bimetallic metal organic framework modified screen printed electrode for simultaneous voltammetric determination of calcium folinate and methotrexate.

BACKGROUND AND PURPOSE: Calcium folinate (CFT) is a medication used as an antidote to methotrexate (MTT) and as a vitamin against anaemia. MTT is used to treat a variety of carcinomas, including acute lymphoblastic leukaemia, head and neck cancer, gastric cancer, breast cancer and choriocarcinoma. EXPERIMENTAL APPROACH: A nanocomposite made of Fe and Mg linked to a 1,4-benzene dicarboxylate ligand metal organic framework (FeMg-BDC MOF) was created for this investigation. Using field emission scanning electron microscopy, the morphology and structure of the Mg-BDC MOF were examined. Next, a screen-printed electrode modified with a synthetic nanocomposite (FeMg-BDC MOF/SPE) was used as the working electrode for voltammetric detection of CFT. KEY RESULTS: The FeMg-BDC MOF/SPE demonstrated high electrocatalytic activity for CFT oxidation as compared to unmodified SPE. The limit of detection is 0.04 μM, and under ideal conditions, the oxidation peak currents of CFT show a linear relationship with its concentration in the range of 0.1 to 390.0 μM. Additionally, when MTT was present, the FeMg-BDC MOF/SPE demonstrated good activity toward CFT determination. The separation of the oxidation peak potentials (255 mV) indicates that differential pulse voltammetry can be used to detect these two medications simultaneously. CONCLUSION: The devised sensor's applicability was confirmed with outstanding results using CFT and MTT assays in pharmaceutical specimens and urine samples.