Single-Cell and Single-Embryo RNA Sequencing.

The scarcity of available donated human embryos for research and technical difficulties present numerous challenges for human development research. Developments in RNA-seq technologies for small inputs, such as single embryos and single cells, now make human applications possible. This chapter outlines single-embryo RNA-seq, with adaptations noted for single-cell RNA-seq and single embryonic/abembryonic compartment applied to preimplantation embryo analysis, from embryo collection to bioinformatic data processing, and the specificities of applications of these techniques in reproductive biology.