Selective preservation of shear-dependent platelet adhesion during microvolume storage in a compact agitation system.

BACKGROUND: Platelet-based applications relying on fresh samples create logistical constraints in research workflows. While the platelet storage lesion (PSL) is well described, its impact on biomechanical platelet function remains unclear, and existing systems are poorly suited to lab-based assays. The digital storage device (DSD) is a compact device designed for room temperature (20-24 °C) microvolume (600 μL) platelet storage under gentle, oscillatory agitation. OBJECTIVES: To assess the capacity for the DSD to preserve platelet biomechanical (shear-dependent) and biochemical functions. METHODS: Platelet-rich plasma (PRP) and washed platelets (WP) were stored for up to 7 days in the DSD or statically at 2 to 6 °C. Shear-dependent platelet adhesion to von Willebrand factor was assessed using a microfluidic model. Biochemical responses were evaluated by TRAP-6-induced PAC-1 activation and ADP-induced aggregation. PSL markers were also measured. RESULTS: Shear-driven adhesion was maintained for 3 days in the DSD (WP: 93% of baseline at day 2, 97% day 3; PRP: 83% day 2, and 74% day 3) and remained GPIb-dependent. Biochemical responsiveness declined, particularly in PRP, where TRAP-6 responses decreased from 52% to 12% after 2 days and ADP aggregation from 60% to 20%. WP retained 84% to 89% TRAP-6 responsiveness over 7 days under microscopy. PSL markers increased alongside progressive glucose consumption. CONCLUSION: The DSD supports shear-dependent platelet adhesion for 3 days of storage in both PRP and WP. In contrast, biochemical responsiveness is not maintained. This divergence highlights that distinct platelet functions are differentially affected during storage, enabling targeted interrogation of biomechanical functions.