Bjørn K. Fabian-Jessing, Anne Louise Askou, Thomas Stax Jakobsen, Rikke L. Adsersen, Anna Bøgh Lindholm, Anne Kirstine Køllner Bjerre, Sidsel Alsing, Bek T, Lars Aagaard, Thomas J. Corydon
suppression and strong PEDF expression were observed. Notably, laser-induced choroidal neovascularization (CNV) was significantly reduced in the therapeutic groups, with the multi-targeting vector achieving the highest level of CNV suppression. Collectively, our data demonstrated robust anti-angiogenic effects of multiple gene therapies, suggesting a "one-and-done" AAV-based delivery of cross-species anti-VEGFA RNAi therapeutics together with PEDF as a valuable tool for the management of neovascular AMD (nAMD) and other complex neovascular ocular diseases.