Lei Nie, Yuanyuan Lu, Yanting Han, Peng Ding
In situ sprayable hydrogel adhesives have emerged as promising biomaterials for biomedical applications, including wound care and as surgical suture alternatives. In this study, a sprayable photopolymerizable hydrogel bio-adhesive was developed using methacrylated hyaluronic acid (HAMA), acrylic acid, and polydopamine (PDA) nanoparticles. A stable hydrogel network was formed by spraying the pre-composite solutions and treating them with ultraviolet (UV) light to achieve rapid polymerization. The fabricated hydrogel bio-adhesives exhibit excellent adhesive properties to various matrices and organs, including the kidney, spleen, liver, stomach, lung, and heart. Moreover, it also exhibits excellent hydrophilicity, swelling properties, and degradability. The adhesive strength of hydrogel bio-adhesives can be improved by increasing the acrylic acid content. The cycled compressive strength and tensile stress tests confirmed the excellent mechanical properties of the fabricated hydrogel bio-adhesives. In addition, the antibacterial experiments revealed that the designed hydrogel bio-adhesives possessed a significant inhibitory effect against E. coli and S. aureus . Fluorescent images and CCK-8 assay confirmed that the hydrogel bio-adhesives exhibited good cytocompatibility. Cell scratch results demonstrated that the hydrogel bio-adhesives could effectively promote cell migration. In summary, this study provided a facile approach to fabricating sprayable hydrogel bio-adhesives, indicating their potential in biomedical applications.