Nucleolar reorganization on stress depends on physicochemical changes due to reduced nascent rRNA synthesis.

Successive maturation of ribosomal subunits occurs through multilayered phase-separated structures of the cell nucleolus. The spatio-functional relationship between transcription of rRNA and nucleolar substructures, and how this adapts to cellular stress remain incompletely understood. In this study, we resolve the sub nucleolar structures using expansion microscopy to reveal ordered structures of fibrillar center (FC) and dense fibrillar component (DFC) domains as nested shells, which are reorganized upon cellular stress like DNA damage or RNA polymerase I (RNAPI) inhibition. Direct visualization of nascent (5' ETS) and mature (28S or 18S) rRNA suggests that rRNA synthesis is a critical regulator of nucleolar size and organization. Nucleolar reorganization upon stress emerges to be a direct function of nascent rRNA levels. Stress-induced transcription inhibition can remodel the sub-nucleolar compartments to a low mobility state and perturbs the nucleolar pH gradient due to the missing rRNA scaffold and other factors. We show that rather than signaling to mediate rDNA repair, nucleolar reorganization arises naturally from reduced rRNA levels and the resultant biophysical restructuring of the nucleolus under cellular stress.