Masato Akiba, Minori Onaga, Tsuyoshi Sekizuka, Nobuki Yoshizawa, Ikuo Uchida
We developed a novel single nucleotide variant (SNV)-based genotyping method for Prototheca bovis based on mitochondrial and plastid genome polymorphisms. Sequencing of five PCR amplicons targeting six informative SNVs discriminated field isolates with a Simpson's D value of 0.927, providing a simple and robust epidemiological typing method.