Systematic approach for revealing biomarkers of diarrheal microbiome of yaks through Metagenomics sequencings.

Yaks are important food ruminants on the Plateau, but the presence of a diarrhea disease is seriously threatening the yak sector. To detect bacterial biomarkers of diarrhea in this animal, metagenomics sequencing of fecal samples from diarrhea (group D) and normal (group H) yaks was performed. The results showed 61963 936 432 and 63 972 070 354 clean samples in the diarrheal and normal yaks, respectively. Genotyping in group D (20 000) was statistically lower than that in group H (80 000) (p<0.05). Firmicutes and bacteroides levels in diarrheal yaks (1.03) were lower than in normal animals. There were 23 phyla and 696 species significantly different between the two yak groups including species of pathogenic Bacteroides fragilis, Alloprevotella tannerae, Parabacteroides merdae, Anaerococcus marasmi, and beneficial Methanobrevibacter millerae, Elusimicrobium minutum, Adlercreutzia equolifaciens. Our results may contribute to the prevention and treatment of diarrheal in yaks in the cold plains areas.