Bioinspired microneedle patch integrating ginger vesicle-silver hybrid nanocomposites with dual antibacterial and angiogenic functions for diabetic wound healing

• Multifunctional microneedle integrating GVs with in situ biomineralized AgNPs had developed for synergistic antibacterial and regenerative wound healing. • The Ag-GV significantly improved AgNPs antimicrobial efficacy, while maintaining the regenerative and biofilms-disrupting properties of GV. • The Ag-GV microneedle enhanced local retention and accelerated diabetic wound healing by eliminating MRSA, disrupting biofilms, and promoting angiogenesis. Chronic diabetic wounds represent a major clinical challenge due to biofilm-forming bacterial infections and impaired angiogenesis, which impedes tissue regeneration and leads to poor therapeutic outcomes. In this study, we developed a multifunctional therapeutic microneedle (Ag-GV/MN) that integrates ginger-derived vesicles (GV) with in situ biomineralized silver nanoparticles (AgNPs) for synergistic antibacterial and regenerative therapy to promote wound healing. GV serves as a biocompatible nanocarrier that not only stabilize and uniformly disperse AgNPs but also contribute inherent antioxidant, anti-inflammatory, and pro-angiogenic properties. The resulting Ag-GV nanocomposite effectively disrupts mature biofilms, eliminates methicillin-resistant Staphylococcus aureus ( MRSA ), and promotes cell migration and vascular regeneration in vitro . When incorporated into a dissolvable microneedle patch, the Ag-GV/MN platform enabled precise, minimally invasive delivery with improved local retention. In a diabetic wound model, this multifunctional Ag-GV/MN significantly reduced bacterial burden, promoted neovascularization and collagen deposition, leading to accelerated wound healing. Overall, this bioactive and antibacterial microneedle platform offers a promising strategy for the treatment of chronic diabetic wounds and have potential for clinical application.