Brain delivery of a neurotrophic peptide derived from secreted amyloid precursor protein APPsα as a therapeutic strategy for Alzheimer's disease

Alzheimer's disease (AD) is characterized by extracellular Amyloid β (Aβ) plaque deposition and the accumulation of hyperphosphorylated Tau species. Whereas neurotoxic Aβ is derived from amyloid precursor protein (APP) by β- and γ-secretase cleavage, APP processing along the non-amyloidogenic pathway results in the secretion of the large neurotrophic ectodomain APPsα. In AD, synapse dysfunction and loss are highly correlated with cognitive decline. However, therapeutic strategies to prevent or reverse synaptic loss remain elusive. Here, we examined the potential of a short C-terminal peptide (CTα16), derived from APPsα to mitigate synaptic deficits in AD model and knockout mice. We tested two different delivery systems and routes of administration: local delivery on the genetic level with AAV9 vectors and nanocarrier-mediated systemic delivery of the peptide. For genetic delivery (AAV-iCTα16), the CTα16 peptide was fused to the pre-pro-domain of thyrotropin-releasing hormone (TRH) leading to processing within the secretory pathway, enabling efficient peptide expression and secretion in vitro and in vivo. When injected into the hippocampus of APP/APLP2-deficient NexCre cDKO mice AAV-iCTα16 fully rescued spine density and synaptic plasticity (LTP), which identifies the CTα16 peptide as a major functional domain of neurotrophic APPsα in vivo. Further, we show that AAV-iCTα16 reverses synaptic impairments of aged THY-Tau22 model mice in the presence of pre-established Tau pathology. For peptide delivery to the brain, we developed CTα16 encapsulating liposomes decorated with either the cell penetrating peptide CycR9 or an α-Transferrin receptor (TfR) antibody for blood-brain barrier (BBB) crossing. Strikingly, intravenous injection of CTα16-liposomes again efficiently restored spine density in aged THY-Tau22 mice to a similar level as AAV-iCTα16. Together, these data suggest that brain delivery of CTα16 can reverse synaptic damage and enhance functional plasticity of the neuronal network and thus holds therapeutic potential for tauopathies in general and AD in particular. • AAV-iCTα16 mediated expression of the CTα16 peptide rescues spine density in APP/APLP2-deficient cDKO mice. • AAV-iCTα16 restores normal synaptic plasticity in APP/APLP2-deficient cDKO mice. • AAV-iCTα16 restores normal spine density in aged transgenic THY-Tau22 mice, an Alzheimer model with Tau pathology. • Blood brain barrier targeted CTα16 containing liposomes mediate efficient peptide uptake into the brain. • Liposomal delivery of CTα16 peptide restores normal spine density in THYTau22 mice.