An RbohC and RbohE-mediated ROS oscillatory circuit regulates root cap turnover in Arabidopsis

. The disruption of these oscillations through chemical inhibitors or exogenous ROS impairs columella initial division and detachment. We identify RbohC and RbohE as major ROS producers, negatively regulated by the NAC transcription factor ANAC33/SOMBRERO. ROS oscillations also affect auxin distribution by modulating the transporter PIN-FORMED2. These findings reveal a redox-based mechanism integrating ROS and auxin signaling to coordinate columella root cap turnover.