Y.F. Li, Natasha Logan, Awanwee Petchkongkaew, Yunhe Hong, Xiaotong Liu, Nicholas Birse, Simon A. Haughey, Terry McGrath, Di Wu, Christopher T. Elliott
The growing vulnerability of the global food supply chain highlights the need for rapid, accurate, and non-destructive authenticity testing. In this study, we developed and validated a workflow integrating Fourier-transform infrared, near-infrared, and X-ray fluorescence spectroscopy with machine learning-based data fusion for black tea authentication. A total of 532 authentic Assam, Darjeeling, Ceylon, and Keemun samples were analysed using five supervised models. A series of information-level, feature-level, and decision-level fusion strategies were developed and compared, with decision-level fusion achieving 100% F1 scores across calibration, validation, and test sets, outperforming individual and other fused methods. The workflow was further applied to 89 commercial teas, identifying a 6.74% non-compliance rate, all from online platforms. This approach eliminates the need for expensive mass spectrometry or stable isotope-based instrumentation and is well suited for accurate, cost-effective food authenticity testing in non-specialist laboratories, particularly in developing countries.