A dual-signals peptide-based probe for fluorometric and colorimetric detection of copper(II) ions and glyphosate in multiple food samples and biological system

A new dual-signals peptide-based probe FAHK was rationally designed and successfully synthesized for sequential detection of Cu 2+ and glyphosate throughfluorescence and colorimetric sensing method. The limit of detections (LODs) for Cu 2+ and glyphosate were calculated to be 49.7 nM and 66.3 nM, respectively. Additionally, FAHK not only demonstrated excellent accuracy in the determination of real food, water and vegetables samples, but also was applied for fluorescence imaging Cu 2+ and glyphosate in two biological systems. Besides, test strips experiments showed that FAHK has extraordinary potential in environmental monitoring and food analysis applications. Meanwhile, we explored the application of FAHK in molecular logic gates, and a visual portable sensing platform was established via the smartphone color Picker App system, successfully achieving semi-quantitative analysis of Cu 2+ and glyphosate. More importantly, FAHK has successfully achieved the detection of glyphosate residues on six food surfaces and soil samples with satisfactory results.