Vibration reduces transcript levels of the Golgi-associated gene GOLGA2 in HeLa cells.

Mechanical vibration induces cytoskeletal remodeling, which may influence organelle-associated gene expression. Because Golgi structure depends on cytoskeletal interactions, we tested whether vibration alters mRNA levels of GOLGA2 , a gene encoding the Golgi matrix protein GM130. Using qRT-PCR, we found that HeLa cells exposed to vibration showed reduced GOLGA2 transcript abundance relative to no vibration controls. In contrast, transcript levels of LMNA , a gene associated with nuclear structure, were not significantly changed. These findings show that vibration reduces mRNA abundance of the Golgi-associated gene GOLGA2 , indicating a selective response relative to LMNA.