Maximilian Haist, Marc-A. Baertsch, Nathan E. Reticker-Flynn, Guolan Lu, Tim Kempchen, Pauline Chu, Gustavo Vazquez, Han Chen, John B. Sunwoo, Weiruo Zhang, Eyiwunmi E. Laseinde, Bonny Adami, S. Zimmer, Justus Kaufman, Quynh Thu Le, Andrew J. Gentles, Christina S. Kong, Sylvia K. Plevritis, Yury Goltsev, John W. Hickey, Garry P. Nolan
Lymph node (LN) colonization in cancer is linked to poor prognosis. Evidence suggests that LN colonization induces systemic immunosuppression, facilitating distant metastasis. We investigated LN-mediated immunosuppression in patients with head-and-neck cancer using spatial proteomics, spatial transcriptomics, and an in vivo model of melanoma LN metastasis. Both primary tumors and paired LNs of nodal-positive patients exhibit enhanced interferon-γ signaling and an enrichment of immunosuppressive myeloid cells and cancer-associated fibroblasts (CAFs). The spatial intersection of these myeloid-CAF-enriched niches with perifollicular T cell zones and LN follicles is linked to enhanced T cell dysfunction and Treg activation therein, thereby driving architectural LN remodeling. These immune suppressive changes extend to adjacent non-tumor-involved LN regions and nearby tumor-free LNs, but were not detected in LNs of non-cancer patients, reflecting a systemic effect that compromises anti-tumor immunity beyond the tumor-involved LN. Hence, our findings establish LN colonization as an active driver of systemic immunosuppression, facilitating metastatic progression.