Harnessing the promiscuity of a nonulosonic acid synthase for the chemoenzymatic synthesis of pseudaminic acid derivatives and specificity mapping of an anti-Pse antibody.
Natasha E Hatton, Niccolay Madiedo Soler, Ethan D Goddard-Borger, Martin A Fascione
原始摘要（英文原文）· Original abstract
Herein we report a divergent route from a single diazido precursor to four 6-deoxy-l-altdiamides, converted on preparative scale by promiscuous C. jejuni PseI into pseudaminic acid derivatives Pse5Ac7Ac, Pse5Ac7Fm, Pse5Ac7Hb and Pse5Hb7Fm. Surface plasmon resonance revealed the 1E8 monoclonal antibody tolerates varied C7 amides but not a C5 3-hydroxybutyryl group.
Harnessing the promiscuity of a nonulosonic acid synthase for the chemoenzymatic synthesis of pseudaminic acid derivatives and specificity mapping of an anti-Pse antibody. — 科研速览 Science Skim