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◆ Methods in enzymology2026-01-01

Histone Decatylase 6 and Tau-induced microglial chemotaxis by trans-well migration and wound-scratch assay.

Subashchandrabose Chinnathambi, Smita Eknath Desale

原始摘要(英文原文)· Original abstract
Development of Alzheimer's is promoted through the accumulation of Tau and Amyloid Beta (Aβ) proteins at various neuronal as well as glial junctions. Tau, a microtubule-associated protein, is localized at the axonal region of neurons under physiological conditions. The main function of Tau protein is to stabilize microtubules, mediated by the electrostatic interaction of their surface with the repeat domains of Tau. The post-translational modifications (PTMs) are necessary for the physiological functioning of protein, but upon abnormal phosphorylation of Tau, Neuro-fibrillary Tangles of protein are generated disrupting normal brain functionality. Cell migration is a physiological functioning of the cell necessary for various signalling from development, cellular communication, immune function etc. cellular microenvironment affect the behaviour of the cell, which can be detected by its migration propensity. The in vitro assays assist in understanding the adhesion, migration and invasion strategies of migratory cells in response to extracellular stimuli. The migration property can be linked to phenotypic changes of microglia as anti-inflammatory phenotype of microglia display increase migration and invasion, hence understanding migration propensity over Tau and HDAC6 exposure is necessary.
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Histone Decatylase 6 and Tau-induced microglial chemotaxis by trans-well migration and wound-scratch assay. — 科研速览 Science Skim