◆ Stem Cell Reviews and Reports2026-08-31· Protocol (science)
From Design to Single-Cell Cloning: A Complete RNP-Based CRISPR-Cas9 Protocol for Precision Gene Correction in Human iPSCs
Giacomo Roman, Knut H. Lauritzen, Barbora Smolkova, Runar Almaas, Per Morten Sandset, Benedicte Stavik, Gareth J. Sullivan, Maria E. Chollet
原始摘要（英文原文）· Original abstract
Summary This integrated end-to-end protocol provides a comprehensive and efficient workflow for the generation, screening and expansion of high-quality monoclonal iPSC lines after CRISPR-Cas9 editing.