Borala Liyanage D Deepali, Caiping Huang, Wei Zhang, Hui Wang, Yuan Wang, Kevin D Hyde, K W Thilini Chethana, Jiye Yan
Cross-kingdom RNA (CK-RNA) communication enhances understanding of inter-kingdom interactions by uncovering RNA-mediated signaling among plants, fungi, bacteria, and viruses through mechanisms like RNA interference (RNAi). This review highlights agricultural applications of CK-RNA, including Spray-Induced Gene Silencing (SIGS), Host-Induced Gene Silencing (HIGS) and Microbe-Induced Gene Silencing (MIGS), which offer environmentally friendly and sustainable alternatives to conventional chemical management. The integration of CK-RNAs as targets and MIGS as delivery methods presents a promising pathway for viable, environmentally friendly crop protection.
Cross-kingdom RNA (CK-RNA) communication is an advanced concept that enhances our understanding of inter-kingdom interactions by uncovering RNA-mediated signaling among plants, fungi, bacteria, and viruses. This review provides a detailed overview of CK-RNA mechanisms, highlighting on plant-microbe interactions. Through RNA interference (RNAi), CK-RNA allows organisms to exchange regulatory signals that influence gene expression, defense responses, metabolism, and adaptive processes across kingdoms. Furthermore, this review highlights agricultural applications of CK-RNA, including Spray-Induced Gene Silencing (SIGS), Host-Induced Gene Silencing (HIGS) and Microbe-Induced Gene Silencing (MIGS) which offer environmentally friendly and sustainable alternatives to conventional chemical management. Overall, the integration of CK-RNAs as target and MIGS as delivery method presents a promising pathway for viable, environmentally friendly crop protection that aligns with modern goals of environmental sustainability and agricultural resilience.