Dracafoliasides A-D, four undescribed spirostanol glycosides from Dracaena angustifolia with nitric oxide production inhibitory activity.

Four undescribed spirostanol glycosides (23S,24S)-spirosta-5,25(27)-dien-1β,3β,23,24-tetrol 1-O-{2,3,4-tri-O-acetyl-α-L-rhamnopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-α-L-arabinopyranoside} 24-O-β-D-quinovopyranoside (1), spirosta-5,25(27)-dien-1β,3β,17α-triol 1-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-xylopyranoside (2), spirosta-5,25(27)-diene-1β,3β,17α-triol 1-O-β-D-glucopyranosyl-(1→3)-O-β-D-xylopyranoside (3), (25R)-spirosta-5-ene-1β,3β,17α-triol 1-O-α-L-rhamnopyranosyl-(1→2)-O-β-D-xylopyranoside (4), together with six known compounds (5-10) were isolated from Dracaena angustifolia roots. Their chemical structures were determined based on extensive spectroscopic analysis, including IR, HR-ESI-MS, 1D and 2D NMR spectra. Compounds 1-3, 5, 7 and 10 significantly inhibited nitric oxide (NO) production in lipopolysaccharide activated RAW264.7 cells with IC50 values ranging from 22.5 to 36.2 µM, while compounds 4 and 9 showed moderate effects with IC50 values of 67.2 and 60.5 µM, respectively.