Analytical characterization, antifungal activity and textile dyeing of blue green pigment produced by Pseudomonas aeruginosa for potential industrial application.

Pseudomonas aeruginosa produces the blue-green phenazine pigment known as pyocyanin, which has shown promise in the fields of health, agriculture, environmental protection, and cosmetics. Because of their natural colour, safety, and inexpensive manufacturing costs, microbial pigments are a promising substitute for natural pigments and offer a wide range of possible applications. The present work focused on the extraction of a blue-green pigment produced by Pseudomonas aeruginosa. The chloroform extraction method was employed to extract the pigment. The yield of total pigment production was 50 mg/ml. The extracted pigment was chemically characterized by UV-vis, HPLC, GC-MS, FTIR. The antifungal properties were assessed using agar well diffusion method and the results showed inhibition against four pathogens (Fusarium oxysporum (15.60 mm), Aspergillus niger (25.60 mm), Rhizoctonia solani (14.30 mm), Magnaporthe oryzae (28.00 mm) at 30 mg/ml concentration followed by 20 mg/ml. The evaluation of the biocolor as a dye was executed on different types of fabrics (velvet, wool, georgette, cotton silk) of 3 × 3 cm which was collected from the local market of Jammu. The dyed fabrics were checked for washing, rubbing, and light fastness and also checked the pigment absorption by fabrics. The antimicrobial properties of dyed fabrics were assessed using Bit diffusion method and the results showed inhibition against four human pathogens (Escherichia coli, Bacillus cereus, Klebsiella pneumonia and Staphylococcus aureus). The highest zone was shown against B. cereus of 15.60 mm (unmordant velvet fabric) and against S. aureus of 20.30 mm (mordant velvet fabric). The potential of bacterial pigments (bio pigments) as sustainable, non-toxic dyeing agents with broad applicability across textile and biomedical domains, offering a promising alternative to synthetic dyes.