Yamilé López-Hernández, Prashanthi Kovur, David S Wishart
The Human Metabolome Database (HMDB) is a web-based resource that supports metabolite identification and interpretation for human studies. This chapter outlines five protocols: (1) identifying metabolites using mass spectrometry with retention time or collision cross-section data (MS-RT/CCS); (2) identifying metabolites using tandem mass spectrometry (MS/MS); (3) identifying metabolites using nuclear magnetic resonance (NMR); (4) comparing measured concentrations to HMDB reference values; and (5) interpreting metabolomics results via HMDB's MetaboCards and PathBank links.