Gang Zhao
This chapter examines the application of microfluidics in cryopreservation, addressing the limitations of conventional techniques and highlighting how microfluidic approaches overcome these challenges. It delves into the fundamental principles of microfluidic cryopreservation, including microscale heat and mass transfer dynamics, precise control of cryoprotective agents (CPAs), and ultra-rapid cooling/warming. The chapter also showcases key applications, including on-chip cell membrane permeability measurement, dynamic CPA addition/removal, and integrated cryopreservation workflows on microfluidic platforms. Finally, it explores the transformative potential of microfluidics in enabling more precise, controllable, and efficient cryopreservation methods, while outlining future research directions and potential technological synergies.