Chemical Characterization and Pharmacological Activity of Byrsonima crassifolia (L.) Kunth (Murici): Antinociceptive Effects, Edema Reduction, and Molecular Docking.

This study investigated the chemical composition, anti-inflammatory and antinociceptive activities of murici leaf (ELM) and bark (EBM) extracts using adult zebrafish (Danio rerio). HPLC analysis identified rutin, isoquercitrin, and kaempferol-3-O-rutinoside as the major phenolic compounds. Both extracts exhibited low toxicity and no sedative effects at the lowest tested doses. ELM showed abdominal antinociceptive activity mediated by TRPA1 and significantly reduced carrageenan-induced abdominal edema. EBM inhibited both corneal (TRPV1) and abdominal (TRPA1) nociception, producing effects similar to morphine. Molecular docking corroborated the in vivo findings, revealing favorable interactions with TRPA1, COX-2, and TRPV1, supported by stable hydrogen-bond formation. The combined chemical, biological, and computational evidence highlights murici as a promising natural source of multifunctional phytotherapeutic agents with antinociceptive activity and potential anti-inflammatory properties.